General description

Native sphingomyelinase from Staphylococcus species. May be applied exogenously to initiate the sphingomyelin signaling pathway. Has very little activity on phosphatidylcholine.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Galve-Roperh, I., et al. 1997. Brain Res. Mol. Brain Res.52, 90.Jarvis, W.D., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 73.Mathias, S., et al. 1993. Science259, 519.Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572.

Packaging

10 u in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 250 mM phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial use, aliquot and refrigerate (4°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of trinitrophenylaminolauryl (TNPAL)-sphingomyelin per min at 37°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)